ANALISIS PERBEDAAN KUALITAS TROMBOSIT METODE PRP-PC DAN APHERESIS DI UTD PMI KOTA SURABAYA

Zainul Arifin; Hasyim As'ari; Fuad Ardiyansyah

doi:10.36526/biosense.v9i2.7481

Authors

Zainul Arifin UTD PMI Kota Surabaya
Hasyim As'ari Program Studi Biologi, FMIPA, Universitas PGRI Banyuwangi
Fuad Ardiyansyah Program Studi Biologi, FMIPA, Universitas PGRI Banyuwangi

DOI:

https://doi.org/10.36526/biosense.v9i2.7481

Keywords:

trombosit konsentrat; PRP-PC; Apheresis; kualitas trombosit; transfusi darah

Abstract

Transfusi trombosit merupakan terapi penting bagi pasien dengan trombositopenia dan gangguan hematologi. Kualitas trombosit konsentrat dipengaruhi oleh metode produksi, yaitu Platelet Rich Plasma–Platelet Concentrate (PRP-PC) dan Apheresis. Penelitian ini bertujuan menganalisis perbedaan kualitas trombosit berdasarkan jumlah trombosit, pH, dan residu leukosit. Penelitian menggunakan desain kuantitatif komparatif dengan pendekatan cross-sectional yang dilaksanakan di UTD PMI Kota Surabaya pada Desember 2025. Sampel terdiri atas 10 unit PRP-PC dan 10 unit trombosit Apheresis, dengan pengujian masing-masing dilakukan tiga kali. Analisis data meliputi uji Shapiro–Wilk, Independent-Sample t-test, dan Mann–Whitney U test pada tingkat signifikansi 0,05. Hasil menunjukkan seluruh produk memenuhi standar mutu nasional sesuai Permenkes RI Nomor 91 Tahun 2015. Rata-rata jumlah trombosit pada PRP-PC sebesar 7,86×10¹⁰/unit dan Apheresis 30,75×10¹⁰/unit; residu leukosit masing-masing 0,04×10⁹/unit dan 0,01×10⁹/unit; serta pH 6,68 dan 7,06. Uji statistik menunjukkan perbedaan yang signifikan pada seluruh parameter mutu (p < 0,05). Disimpulkan bahwa metode Apheresis menghasilkan trombosit dengan kualitas lebih unggul dibandingkan PRP-PC dari aspek jumlah trombosit, kemurnian produk, dan kestabilan pH, sehingga lebih direkomendasikan untuk kebutuhan transfusi klinis.

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