IDENTIFIKASI MORFOLOGI SPERMA KATAK FAMILI (BUFONIDAE) MENGGUNAKAN METODE GALLI MAININI:

Fuad  Ardiyansyah; Dini Endah Tri Kusumawati; Joko Prasetyo; Ria Rafidha Kasyim; Hasyim As'ari

doi:10.36526/biosense.v7i02.3981

Fuad Ardiyansyah Program Studi Biologi, FMIPA, Universitas PGRI Banyuwangi
Dini Endah Tri Kusumawati Prodi Biologi, Fakultas MIPA, Universitas PGRI Banyuwangi
Joko Prasetyo Program Studi Biologi, Fakultas MIPA, Universitas PGRI Banyuwangi
Ria rafidha kasyim Prodi Biologi, Fakultas MIPA, Universitas PGRI Banyuwangi
Hasyim As'ari Program Studi Biologi, FMIPA, Universitas PGRI Banyuwangi

DOI: https://doi.org/10.36526/biosense.v7i02.3981

Keywords: Morphological identification; Frog; Sperm

Abstract

Frogs and toads belong to the same order, Anura, but toads usually have rough and bumpy skin like those in the Bufonidae family. This study aims to study the morphological characteristics of male frog sperm cells using the galli mainini method, using pregnant women's urine containing the hormone human Chorionic Gonadotropin (hCG) to stimulate sperm production. Urine is injected into the frog's abdominal membrane, and after 30 minutes, the frog is stimulated to excrete urine containing sperm. The results showed that the length of Bufonidae frog spermatozoa ranged from 32.54 μm to 73.31 μm with an average of 56.35 μm. The acrosome and tail of the spermatozoa are almost the same length, making these two parts difficult to distinguish. In conclusion, optimization of the dose and concentration of the hCG hormone is needed for further research and exploration of its long-term effects on male frogs.

References

Addaha, H., Hon Tjong, D., & Wilson Novarino. (2015). Variasi Morfologi Katak Pohon Bergaris Polypedates leucomystax Gravenhorst, 1829 (Anura; Rhacophoridae) di Sumatera Barat Morfological Variation of Striped Tree Frog Polypedates leucomystax Gravenhorst, 1829 (Anura; Rhacophoridae) in West Sumatera. Online Jurnal of Natural Science, 4(3), 348–354.
Amin, B. (2020). Katak Di Jawa Timur. Angewandte Chemie International Edition, 6(11), 108.
Ardhani, F. (2018). Karakteristik Morfologik dan Morfometrik Spermatozoa Ayam Nunukan. Jurnal Peternakan, 15(2), 62. https://doi.org/10.24014/jupet.v15i2.4368
Nesty, R., Djong, H. T., & Henny, H. (2013). Variasi Morfometrik Kodok Duttaphrynus melanostictus (Schneider, 1799) (Anura: Bufonidae) di Sumatera Barat yang Dipisahkan oleh Bukit Barisan Morphometric variations of toad Duttaphrynus melanostictus (Schneider, 1799) (Anura: Bufonidae) in West Sumatera. Jurnal Biologi Universitas Andalas (J. Bio. UA.), 2(1), 37–42. http://jbioua.fmipa.unand.ac.id/index.php/jbioua/article/view/36
Putri, A. R. I., Kurniawan, N., & Marhendra, A. P. W. (2013). Pengaruh Hormon Hipofisa dan Ovaprim Terhadap Ovulasi Serta Pengaruh Pakan Terhadap Pertumbuhan Berudu Katak Fejervarya cancrivora. Biotropika, 1(5), 191–195. http://www.biotropika.ub.ac.id/index.php/biotropika/article/view/178

Rianto, A., & Darmawan, A. (2022). Keanekaragaman Amfibi pada Lahan Agroforestry di Pekon Kotabatu, Tanggamus, Lampung. Journal of Forest Science Avicennia, 5(1), 58–70. https://doi.org/10.22219/avicennia.v5i1.19941
Siahaan, K., Dewi, B. S., & Darmawan, A. (2019). The Diversity of Amphibian from Order Anura in the Protected and Utilization Blocks of Integrated Educational Conservation Forest, Wan Abdul Rachman Great Forest Park. Jurnal Sylva Lestari, 7(3), 370. https://doi.org/10.23960/jsl37370-378
Yudha, D. S., Eprilurahman, R., Trijoko, Alawi, M. F., & Tarekat, A. A. (2014). Keanekaragaman Jenis Katak Dan Kodok (Ordo Anura) Di Sepanjang Sungai Opak Propinsi Daerah Istimewa Yogyakarta. Jurnal Biologi, 18(2), 52–59.

IDENTIFIKASI MORFOLOGI SPERMA KATAK FAMILI (BUFONIDAE) MENGGUNAKAN METODE GALLI MAININI

KARAKTERISTIK MORFOLOGI SPERMATOZOA KATAK FAMILI BUFONIDAE

Abstract

References

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