POTENSI ISOLAT Pseudomonas BERFLUORESEN DALAM MENGHASILKAN ASAM SIANIDA (HCN) UNTUK APLIKASI AGENS HAYATI
DOI:
https://doi.org/10.36526/biosense.v9i1.6804Keywords:
Kata kunci: Pseudomonas berfluoresen, HCN, agens hayati, spektrofotometer.Abstract
Penelitian ini bertujuan untuk menilai kemampuan beberapa isolat Pseudomonas berfluoresen dalam menghasilkan asam sianida (HCN) sebagai indikator potensi agens hayati. Lima isolat diuji, yaitu Pf Cas 3, Pf Cas, Pf LAH T2, Pf LAH, dan Pf LAH S1, serta satu kontrol tanpa inokulum. Pengujian dilakukan menggunakan indikator asam pikrat–Na₂CO₃ dan pengukuran nilai absorbansi dengan spektrofotometer pada panjang gelombang 625 nm. Hasil menunjukkan bahwa isolat Pf Cas 3, Pf Cas, dan Pf LAH T2 menghasilkan HCN dengan nilai absorbansi masing-masing 0,021; 0,014; dan 0,012. Sementara Pf S32 dan Pf LAH S1 menunjukkan hasil negatif dengan nilai absorbansi rendah (0,003 dan 0,002). Kontrol menunjukkan nilai 0,00. Isolat Pf Cas 3 dikategorikan paling potensial untuk dikembangkan sebagai agens hayati penghasil HCN dalam mendukung sistem pertanian berkelanjutan.
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