GREEN SYNTHESIS DAN UJI AKTIVITAS ANTIOKSIDAN NANOPARTIKEL EMAS MENGGUNAKAN BIOREDUKTOR EKSTRAK DAUN SALAM (Syzygium polyanthum (Wight) Walp.)
DOI:
https://doi.org/10.36526/jc.v8i2.9377Keywords:
Gold Nanoparticle, Green Synthesis, Indonesian Bay LeafAbstract
Nanopartikel emas (AuNPs) adalah salah satu jenis nanopartikel logam yang berukuran 1-100 nm yang dapat diaplikasikan dalam berbagai bidang. Sintesis nanopartikel emas dapat dilakukan dengan beberapa metode. Penelitian ini dilakukan dengan tujuan untuk melakukan sintesis dan karakterisasi nanopartikel emas dengan metode green synthesis menggunakan bioreduktor ekstrak daun salam (Syzygium polyanthum (Wight) Walp.) serta untuk mengetahui aktivitas antioksidan nanopartikel emas hasil sintesis menggunakan metode ABTS dengan variasi konsentrasi 20; 10; 5; dan 2,5 ppm. Karakterisasi dilakukan dengan menggunakan instrument spektrofotometer UV-vis untuk mengetahui panjang gelombang maksimum dan absorbansi serta TEM untuk mengetahui ukuran partikel AuNPs hasil sintesis. Diperoleh hasil bahwa nanopartikel emas dapat terbentuk ditandai dengan perubahan warna menajdi ungu dengan konsentrasi 20 ppm berada panjang gelombang maksimum 540 nm dengan absorbansi 0,475 nm serta ukuran partikel dengan rentang 10-16 nm dan didapatkan rata-rata ukuran partikel sebesar 13,6. Nanopartikel emas hasil sintesis juga menunjukan aktivitas antioksidan yang sangat kuat dengan nilai inhibisi 73,9% (20 ppm); 58,5% (10 ppm); 38,8% (5 ppm); dan 35,2% (2,5 ppm) dengan nilai IC50 sebesar 2,4 µg/mL.
Gold nanoparticles (AuNPs) are a type of metallic nanoparticle with sizes ranging from 1 to 100 nm and have wide-ranging applications in various fields. Gold nanoparticles can be synthesized using several methods. This study aimed to synthesize and characterize gold nanoparticles through a green synthesis approach using Syzygium polyanthum (Wight) Walp. (Indonesian bay leaf) extract as a bioreducing agent and to evaluate the antioxidant activity of the synthesized gold nanoparticles using the ABTS assay at concentrations of 20, 10, 5, and 2.5 ppm. Characterization was performed using UV–Vis spectrophotometry to determine the maximum absorption wavelength and absorbance, while transmission electron microscopy (TEM) was used to determine the particle size of the synthesized AuNPs. The results demonstrated the successful formation of gold nanoparticles, indicated by a color change to purple. At a concentration of 20 ppm, the synthesized AuNPs exhibited a maximum absorption wavelength of 540 nm with an absorbance of 0.475. TEM analysis revealed particle sizes ranging from 10 to 16 nm, with an average particle size of 13.6 nm. Furthermore, the synthesized gold nanoparticles exhibited very strong antioxidant activity, with inhibition values of 73.9% (20 ppm), 58.5% (10 ppm), 38.8% (5 ppm), and 35.2% (2.5 ppm), and an IC₅₀ value of 2.4 µg/mL.
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