GREEN SYNTHESIS NANOPARTIKEL EMAS MENGGUNAKAN EKSTRAK DAUN ALPUKAT (PERSEA AMERICANA MILL.) SEBAGAI BIOREDUKTOR SERTA UJI AKTIVITAS ANTIOKSIDANNYA
DOI:
https://doi.org/10.36526/jc.v8i2.9241Keywords:
Gold nanoparticles, green synthesis, avocado leaves, antioxidants, ABTSAbstract
Gold nanoparticles (AuNPs) are materials ranging in size from 1 to 100 nm that possess unique optical, chemical, and biological characteristics, making them potentially useful in various fields, including as antioxidants. The use of toxic chemical reagents in conventional synthesis has driven the development of more environmentally friendly methods through green synthesis. This study aims to produce gold nanoparticles using an avocado leaf (Persea americana Mill.) infusion extract as a bioreductant and to evaluate their antioxidant activity. Synthesis was performed using a 20 ppm HAuCl₄ solution, and the resulting AuNPs were characterized using a UV-Vis spectrophotometer and Transmission Electron Microscopy (TEM). Antioxidant activity was analyzed using the ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate)) radical scavenging assay at concentrations of 2.5, 5, 10, and 20 ppm. The data were analyzed based on the percentage of scavenging (% inhibition) and IC₅₀ using linear regression. The results of the study showed that AuNP synthesis was successful, as indicated by a purplish-red color change in the solution and the appearance of a Surface Plasmon Resonance (SPR) peak at a maximum wavelength of 537 nm with an absorbance value of 0.336. TEM characterization revealed an average particle diameter of 30.91 nm. Antioxidant activity increased linearly with increasing AuNPs concentration, with percentage inhibition values of 39.2%, 53.3%, 66.2%, and 89.6%, respectively. Regression analysis yielded an IC₅₀ value of 4.93 ppm, indicating that AuNPs produced via green synthesis using avocado leaf extract exhibit very strong antioxidant activity.
References
Aini, F.Q. And Taufikurohmah, T. (2022) ‘The Effect Of Nanogold - Nanosilver Injection On Increasing The Immunity Of Community Affected By Covid-19’, International Journal Of Current Science Research And Review, 05(04), Pp. 1116–1125. Available At: Https://Doi.Org/10.47191/Ijcsrr/V5-I4-34.
Andry, M. Et Al. (2025) ‘Antioxidant Activity Test Of Avocado Leaf Kombucha ( Persea Americana Mill .) Based On Fermentation Duration Using The Abts Method’, Journal Of Pharmaceutical And Sciences, 8(4), Pp. 2609–2622.
Anggorowati, D.A., Priandini, G. And Thufail (2016) ‘Potensi Daun Alpukat Sebagai Minuman Herbal Yang Kaya Antioksidan’, Industri Inovatif, 6(1), Pp. 1–7. Available At: Https://Ejournal.Itn.Ac.Id/Index.Php/Industri/Article/View/912.
Aprilia, N.R. And Taufikurohmah, T. (2024) ‘Jurnal Kimia Sains Dan Aplikasi Green Synthesis Of Gold Nanoparticles Using Basella Alba Leaf Extract And Their Antioxidant Activity’, Journal Of Scientific And Applied Chemistry, 27(8), Pp. 381–387.
Aryanti, R., Perdana, F. And Rizkio, R. (2018) ‘Telaah Metode Pengujian Aktivitas Antioksidan Pada Daun Teh Hijau ( Camellia Sinensis ( L .) Kuntze ) Study Of Antioxidan Activity Testing Methods Of Green Tea ( Camellia Sinensis ( L .) Kuntze )’, Jurnal Surya Medika, 7(1), Pp. 15–24. Available At: Doi: Https:/Doi.Org/10.33084/Jsm.Vxix.Xxx.
Dinh, T. Et Al. (2025) ‘A Review Of Gold Nanoparticle Synthesis: Transitioning From Conventional Techniques To Plant-Mediated Green Nanofabrication’, Materials Today Chemistry, 48(August), Pp. 1–26. Available At: Https://Doi.Org/10.1016/J.Mtchem.2025.103003.
Farihah, N.I. And Taufikurohmah, T. (2024) ‘Green Synthesis Gold Nanoparticles Using Bioreductant Red Shoot Leaf Extract ( Syzygium Myrtifolium Walp .) And Activity As Antioxidant’, Jurnal Pijar Mipa, 19(4), Pp. 746–752.
Fatima, Z. Et Al. (2024) ‘Biocatalysis And Agricultural Biotechnology Green Synthesis , Properties , And Biomedical Potential Of Gold Nanoparticles : A Comprehensive Review’, Elsevier, 59(103271), Pp. 1878–8181.
Fazrin, E.I. Et Al. (2020) ‘Review : Sintesis Dan Karakterisasi Nanopartikel Emas ( Aunp ) Serta Konjugasi Aunp Dengan Dna Dalam Aplikasi Biosensor Elektrokimia’, Pendipa Journal Of Science Education, 4(2), Pp. 21–39.
Fernanda, C.D. And Taufikurohmah, T. (2024) ‘Green Synthesis Gold Nanoparticles Using Bioreductor Of Butterfly Pea (Clitoria Ternatea L.) Leaf Extract As An Antioxidant’, Jurnal Pijar Mipa, 19(4), Pp. 726–731.
Gerung, J.B. Et Al. (2025) ‘Formulasi Serum Antiaging Nanopartikel Emas Dengan Fraksi Daun Sirih Hutan Gambut Kalimantan ( Piper Aduncum )’, Sasambo Journal Of Pharmacy, 6(1), Pp. 1–6.
Ilyas, M. Et Al. (2024) ‘Penyuluhan Pembuatan Infusa Tanaman Obat Di Desa Puso Jaya Kecamatan Konda Kabupaten Konawe Selatan’, Jurnal Abdi Dan Dedikasi Kepada Masyarakat Indonesia (Nadikami), 02(1), Pp. 20–30.
Indriyani, R. And Taufikurohmah, T. (2024) ‘Green Synthesis Of Gold Nanoparticles Using Moringa Oleifera Leaf Extract Bioreductor ( Moringa Oleifera L .) And Activity Test As Antioxidant’, Jurnal Pijar Mipa, 19(5), Pp. 881–887.
Lestari, G., Cahyadi, K. And Suprihatin, I. (2021) ‘Characterization Of Gold Nanoparticles From Clove Flower Water Extract And Its Antioxidant Activity’, Jurnal Sains Materi Indonesia, 22(2), Pp. 93–100.
Nadhifah, A. And Mulia, M. (2025) ‘Uji Kualitatif Fitokimia Ekstrak Daun Alpukat (Persea Americana Mill.) Dan Daun Belimbing Wuluh (Averrhoa Bilimbi L.) Serta Kombinasinya’, Jurnal Pendidikan Dan Sains, 5(5), Pp. 2487–2496.
Oktavia, I.N. And Sutoyo, S. (2021) ‘Article Review: Synthesis Of Silver Nanoparticles Using Bioreductor From Plant Extract As An Antioxidant’, Unesa Journal Of Chemistry, 10(1), Pp. 37–54.
Paramita, S.A. And Priyono (2024) ‘Perkembangan Nanosains Dan Nanoteknologi: Fenomena Dari Sifat Fisika Dan Kimia’, Berkala Fisika, 27(2), Pp. 49–57.
Pertiwi, R.D., Utami, T.P. And Michelle (2024) ‘Biosintesis Dan Uji Antioksidan Nanopartikel Emas Menggunakan Kuersetin’, Archives Pharmacia, 6(2), Pp. 99–116.
Putri, C. And Edityaningrum, C. (2025) ‘Optimasi Kombinasi Polimer Hidroksi Propil Metil Selulosa K4m Dan Natrium Karboksimetil Selulosa Pada Formula Patch Ekstrak Daun Alpukat ( Persea Americana Mill .) Dengan Metode Simplex Lattice Design’, Mpi (Media Pharmaceutica Indonesia), 7(2), Pp. 283–299. Available At: Https://Doi.Org/10.24123/Mpi.V7i2.8002.
Rahmah, R. Et Al. (2023) ‘Phytochemical Screening And Antioxidant Activity Testing Of Avocado Leaf Ethanol Extract (Persea Americana Mill.) Using The Dpph Method’, Journal Of Pharmaceutical And Sciences, 1(1), Pp. 9–25.
Siregar, M. (2018) ‘Uji Aktivitas Antioksidan Infusa Daun Alpukat ( Persea Americana Miller ) Dengan Metode Dpph’, Herbal Medicine Journal, 1(1), Pp. 10–14.
Triana, A.D. And Taufikurohmah, T. (2024) ‘Green Synthesis Of Gold Nanoparticles Using Bioreductors Of Bilimbi Fruit Extract ( Averrhoa Bilimbi L .) As An Antioxidant’, Jurnal Pijar Mipa, 19(5), Pp. 875–880.
Waruwu, A., Ewiantini, N. And Sari, N. (2023) ‘Specific And Non-Specific Standardization Test Of Avocado Leaf Infusa And Decocta’, Jurnal Media Sains, 7(1), Pp. 1–11.
Widarta, I.W. And Wiadnyani, A.A. (2019) ‘Pengaruh Metode Pengeringan Terhadap Aktivitas Antioksidan Daun Alpukat’, Jurnal Aplikasi Teknologi Pangan, 8(3), Pp. 80–85. Available At: Https://Doi.Org/10.31596/Cjp.V3i1.43.
Widarta, I.W.R. And Arnata, I.W. (2017) ‘Ekstraksi Komponen Bioaktif Daun Alpukat Dengan Bantuan Ultrasonik Pada Berbagai Jenis Dan Konsentrasi Pelarut’, Agritech, 37(2), Pp. 148–157. Available At: Https://Doi.Org/10.22146/Agritech.10397.
Yulinar, F. And Suharti, P.H. (2022) ‘Seleksi Proses Ekstraksi Daun Sirih Pada Pra Rancangan Pabrik Hand Sanitizer Daun Sirih Dengan Kapasitas Produksi 480 Ton/Tahun’, Distilat, 8(1), Pp. 146–153.
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