PERBANDINGAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN COCOK BEBEK (Kalanchoe pinnata) DENGAN METODE DPPH DAN ABTS
DOI:
https://doi.org/10.36526/jc.v8i1.7746Keywords:
antioxidant, DPPH, ABTS, cocor bebekAbstract
Kalanchoe pinnata leaves are known as a medicinal plant containing secondary metabolites with potential antioxidant activity. This study aimed to compare the antioxidant activity of the ethanol extract of Kalanchoe pinnata leaves using DPPH and ABTS methods. Extraction was carried out by maceration using ethanol as a solvent from 50 g of dried leaf simplicia, yielding 7.2 g of thick extract with a yield of 14.4%. Antioxidant activity was evaluated at concentrations of 20, 40, 60, 80, and 100 ppm. The results showed that the percentage of inhibition increased with increasing extract concentration in both methods. In the DPPH method, the highest inhibition value was 51.1605% at 100 ppm, while in the ABTS method it was 47.7203%. The IC₅₀ values obtained were 93.54 ppm for the DPPH method and 103.01 ppm for the ABTS method. Based on these values, the antioxidant activity of the ethanol extract of Kalanchoe pinnata leaves was categorized as strong in the DPPH method and moderate in the ABTS method. In conclusion, the DPPH method showed higher antioxidant activity compared to the ABTS method. The ethanol extract of Kalanchoe pinnata leaves has the potential to be developed as a natural antioxidant source in pharmaceutical and functional food applications.
References
Alim, N. et al. (2022) ‘Phytochemical Screening, Relationship of Total Phenolic with Antioxidant Activity Of Ethanol and Methanol Extracts of Kesambi (Schleichera oleosa (Lour.) Oken) Bark’, Jurnal Ilmiah Sains, 22(2), p. 118. Available at: https://doi.org/10.35799/jis.v22i2.40091.
Eisa, N. et al. (2025) ‘Phytochemical Profiling , Antimicrobial , and Antioxidant Activities of Tamarindus indica Pulp Extracts : A Comprehensive Evaluation’, 14(1), pp. 51–56. Available at: https://doi.org/10.14421/biomedich.2025.141.51-56.
Indriyanti, N. and Garmana, A.N. (2011) ‘Ekstrak Daun Cocor Bebek (Kalanchoe pinnata) untuk Terapi Preventif Lupus pada Mencit yang Diinduksi dengan 2,6,10,14 Tetramethylpentadecane’, Journal of Tropical Pharmacy and Chemistry, 1(3), pp. 221–226. Available at: https://doi.org/10.25026/jtpc.v1i3.32.
Jiangseubchatveera, N. et al. (2023) ‘Phytochemicals and Antioxidant Activities of Red Oak, Red Coral and Butterhead’, Tropical Life Sciences Research, 34(1), pp. 1–17. Available at: https://doi.org/10.21315/tlsr2023.34.1.1.
Lestari, N. et al. (2022) ‘Efektivitas Ekstrak Daun Cocor Bebek (Kalanchoe pinnata) Terhadap Peningkatan Jumlah Sel Fibroblas Soket Paska Pencabutan Gigi Tikus Wistar’, Sinnun Maxillofacial Journal, 4(02), pp. 94–103. Available at: https://doi.org/10.33096/smj.v4i02.86.
Lestari, S. et al. (2024) ‘Kandungan Flavonoid dan Aktivitas Antioksidan Kitolod (Hippobroma longiflora L.) pada Ketinggian Tempat Tumbuh Berbeda’, LenteraBio : Berkala Ilmiah Biologi, 13(2), pp. 212–218. Available at: https://doi.org/10.26740/lenterabio.v13n2.p212-218.
Mahardika, R.G., Roanisca, O. and Sari, F.I.P. (2020) ‘Fenolik Total Fraksi Etil Asetat Daun Pelawan (Tristaniopsis merguensis Grifft.)’, Jurnal Sains dan Edukasi Sains, 3(1), pp. 8–14. Available at: https://doi.org/10.24246/juses.v3i1p8-14.
Musa, W.J.A., Bialangi, N. and Kilo, A.K. (2025) ‘Evaluation of Polyphenolic Content , Antioxidant and Anti-diabetic Activity of Different Solvent Extracts of Sauauria vulcani Korth . Leaves’, 24(2), pp. 1–12.
Purwanti, R. and Nur Kholifah, D. (2025) ‘KARAKTERISTIK SIMPLISIA DAN SKRINING FITOKIMIA SENYAWA ALKALOID, FLAVONOID, TANIN, DAN SAPONIN EKSTRAK ETANOL DAUN COCOR BEBEK (Kalanchoe pinnata (Lam) Pers.)’, Jurnal Permata Indonesia, 17(1), pp. 1–8. Available at: https://doi.org/10.59737/jpi.v17i1.346.
Putri, A.H., Putriyana, R.S. and Silviani, N. (2019) ‘Isolasi dan Ekstraksi Kelompok Senyawa Flavonoid dari Ekstrak Daun Cocor Bebek (Kalanchoe pinnata)’, Fullerene Journal of Chemistry, 4(2), p. 28. Available at: https://doi.org/10.37033/fjc.v4i2.52.
Sahidin, I. et al. (2023) ‘Phytochemical Profile and Biological Activities of Ethylacetate Extract of Peanut (Arachis hypogaea L.) Stems: In-Vitro and In-Silico Studies with Bibliometric Analysis’, Indonesian Journal of Science and Technology, 8(2), pp. 217–242. Available at: https://doi.org/10.17509/ijost.v8i2.54822.
Sharma, A. et al. (2023) ‘Greener approach for the isolation of oleanolic acid from Nepeta leucophylla Benth. Its derivatization and their molecular docking as antibacterial and antiviral agents’, Heliyon, 9(8), p. e18639. Available at: https://doi.org/10.1016/j.heliyon.2023.e18639.
Situmeang, B. et al. (2022) ‘Aktivitas Antioksidan dan Antibakteri dari Fraksi Ekstrak Metanol Kulit Batang Kesambi (Shleichera Oleosa)’, Jurnal Kimia, 16(1), pp. 53–59. Available at: https://doi.org/10.24843/jchem.2022.v16.i01.p07.
Situmeang, B. et al. (2024) ‘Perbandingan Aktivitas Antioksidan Ekstrak Etil asetat dan Metanol Daun Tumbuhan Matoa (Pometia pinnata)’, Jurnal Penelitian Sains, 26(1), p. 65. Available at: https://doi.org/10.56064/jps.v26i1.919.
Situmeang, B., Swasono, R.T. and Raharjo, T.J. (2025) ‘Evaluation of phytochemical composition, antioxidant, cytotoxic and in silico studies of ethyl acetate fractions of Tristaniopsis merguensis leaves’, Toxicology Reports, 14(January), p. 101911. Available at: https://doi.org/10.1016/j.toxrep.2025.101911.
Warinthip, N. et al. (2023) ‘Chemical Constituents Antioxidant and Antibacterial Activities of the Leaves and Flowers from Gardenia carinata Wallich’, Natural and Life Sciences Communications, 22(1). Available at: https://doi.org/10.12982/NLSC.2023.003.
Yakoubi, R. et al. (2021) ‘Photoprotective, antioxidant, anticholinesterase activities and phenolic contents of different Algerian Mentha pulegium extracts’, Biocatalysis and Agricultural Biotechnology, 34(May), p. 102038. Available at: https://doi.org/10.1016/j.bcab.2021.102038.
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Boima Situmeang, Weny JA Musa, Nurhayati Bialangi, Nani Yulianti
This work is licensed under a Creative Commons Attribution 4.0 International License.
