UJI AKTIVITAS BROMELIN DARI ANANAS COMOSUS DAN PAPAIN DARI CARICA PAPAYA TERHADAP PH MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS

Authors

  • Zahrotun Nafisah Program Studi Kimia, FMIPA, Universitas Palangka Raya https://orcid.org/0009-0003-3283-5176
  • Dermawan Saro Halawa Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya, Jalan Yos Sudarso, Kota Palangka Raya, Kalimantan Tengah 73112
  • Eka Miranda Silaban 1Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya, Jalan Yos Sudarso, Kota Palangka Raya, Kalimantan Tengah 73112
  • Sonia Tri Mart Hutapea 1Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya, Jalan Yos Sudarso, Kota Palangka Raya, Kalimantan Tengah 73112
  • Nasa Natalia 1Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya, Jalan Yos Sudarso, Kota Palangka Raya, Kalimantan Tengah 73112
  • Nia Hana Pratiwi 1Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya, Jalan Yos Sudarso, Kota Palangka Raya, Kalimantan Tengah 73112
  • Kamelia Fahmiati 1Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya, Jalan Yos Sudarso, Kota Palangka Raya, Kalimantan Tengah 73112

DOI:

https://doi.org/10.36526/jc.v8i1.7624

Keywords:

bromelin, papain, activity test, spectrophotometer, pH variation

Abstract

Protease enzymes such as bromelain from Ananas comosus and papain from Carica papaya are cysteine ​​protease enzymes that have great potential in various applications in the food, pharmaceutical, and biotechnology industries. This study aims to characterize the activity of bromelain and papain enzymes against pH variations using a casein-based spectrophotometric method and a tyrosine standard curve. Enzyme activity is determined based on the amount of tyrosine produced from casein hydrolysis and measured at a wavelength of 280 nm. One unit of enzyme activity is defined as the amount of enzyme that produces 1 µmol of tyrosine per mL per unit reaction time. The yields of bromelain and papain enzyme extraction in this study reached 38% and 32% with protein levels of 10.04 and 21.89 mg/mL, respectively. The activity values ​​of bromelain and papain are significantly affected by changes in pH. Bromelain showed optimum activity at pH 6, amounting to 25.4 U/mL, while the activity decreased drastically at acidic pH. The papain enzyme also has an optimum activity of 17.1 U/mL at pH 6, which decreases drastically at alkaline pH. This activity pattern is consistent with the general characteristics of cysteine ​​protease enzymes, which are sensitive to changes in the ionization of their active groups. The differences in response to pH variations indicate the specific characteristics of each enzyme, influenced by the structure and conformational stability of its protein. The results of this study provide important information regarding the optimum working conditions of both enzymes as a basis for developing industrial applications based on local biological resources.

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Published

2026-03-31

How to Cite

Nafisah, Z., Halawa, D. S., Silaban, E. M., Hutapea, S. T. M., Natalia, N., Pratiwi, N. H., & Fahmiati, K. (2026). UJI AKTIVITAS BROMELIN DARI ANANAS COMOSUS DAN PAPAIN DARI CARICA PAPAYA TERHADAP PH MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS. Jurnal Crystal : Publikasi Penelitian Kimia Dan Terapannya, 8(1), 170–178. https://doi.org/10.36526/jc.v8i1.7624

Issue

Vol. 8 No. 1 (2026): Literasi Artikel Penelitian Kimia

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Articles

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Copyright (c) 2026 Zahrotun Nafisah, Dermawan Saro Halawa, Eka Miranda Silaban, Sonia Tri Mart Hutapea, Nasa Natalia, Nia Hana Pratiwi, Kamelia Fahmiati

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This work is licensed under a Creative Commons Attribution 4.0 International License.

 

Creative Commons License Creative Commons Attribution-4.0 International License