MODIFIKASI KITOSAN MENGGUNAKAN SUKROSA DAN GLUKOSA DAN APLIKASINYA SEBAGAI ANTIBAKTERI
DOI:
https://doi.org/10.36526/jc.v8i2.7495Keywords:
Chitosan, Glucose, Antibacterial TestAbstract
Chitosan has antibacterial properties that can inhibit the growth of pathogenic bacteria and microorganisms including Staphylococcus aureus and Escherichia coli. Chitosan in inhibiting bacterial growth is still not optimal, so modifications are needed. This study aims to modify chitosan by adding sucrose and glucose into chitosan with FTIR analysis, physical and chemical properties test, and determine antibacterial activity on gram-positive and negative bacteria. The result of physical properties test shows chitosan is white, odourless and in the form of fine powder, meanwhile sucrose modified chitosan is pale yellow, smells typical of sugar, in the form of fine powder slightly rough and lastly the glucose modified chitosan is rough and sticky powder, smells typical of sugar, and brownish yellow. Then, the solubility test of sucrose-modified chitosan and glucose-modified chitosan is higher than the unmodified chitosan. pH test results in sucrose-modified chitosan and glucose-modified chitosan have a pH of 4-5. FTIR characterization of sucrose-modified chitosan shows a new compound, named NH and the loss of OH groups which indicates the presence of new compounds that bind to sucrose-modified chitosan. The results of antibacterial assay is all samples had inhibition zones, the largest inhibition zone was found in sucrose-modified chitosan on Escherichia coli. Based on the result, sucrose and glucose modified chitosan can be applied as antibacterial agent.
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