MASS BALANCE ANALYSIS OF GLUCOSE FROM SUGARCANE BAGASSE THROUGH ULTRASONIC ENZYMATIC HYDROLYSIS
DOI:
https://doi.org/10.36526/jc.v8i1.6135Keywords:
enzymatic hydrolysis, glucose production, sugarcane bagasseAbstract
This study explores the conversion of sugarcane bagasse into glucose through an enzymatic ultrasonic hydrolysis process. Initially, the bagasse undergoes delignification using 6% sodium hydroxide to remove lignin and free cellulose for further processing. The delignified bagasse is then hydrolyzed using cellulase enzyme under ultrasonic conditions. The results indicate that glucose production is influenced by factors such as raw material preparation, enzyme activity, temperature, and pH. Mass balance calculations were applied to assess the input and output of materials throughout the delignification and hydrolysis processes. The findings show that 10 grams of delignified sugarcane bagasse yielded 5.955 grams of glucose, demonstrating the potential of sugarcane bagasse as a source of bioenergy through glucose production. These results support the utilization of agricultural waste in bioethanol production and contribute to sustainable energy development.
References
Ajala, E. O. et al. (2021) ‘Sugarcane bagasse: a biomass sufficiently applied for improving global energy, environment and economic sustainability’, Bioresources and Bioprocessing. Springer Science and Business Media Deutschland GmbH. doi: 10.1186/s40643-021-00440-z.
Amorim, H. V. et al. (2011) ‘Scientific challenges of bioethanol production in Brazil’, Applied Microbiology and Biotechnology, pp. 1267–1275. doi: 10.1007/s00253-011-3437-6.
Anisya, M., Fitra Andriana, Y. and Islamsyah, H. (2020) ‘Eksplorasi Limbah Ampas Tebu (Bagasse) untuk Material Produk Ecofashion’, Jurnal IKRA-ITH Humaniora, 4(3), pp. 235–243.
de Araujo Guilherme, A. et al. (2019) ‘Ethanol production from sugarcane bagasse: Use of different fermentation strategies to enhance an environmental-friendly process’, Journal of Environmental Management, 234, pp. 44–51. doi: 10.1016/j.jenvman.2018.12.102.
Asgher, M., Ahmad, Z. and Iqbal, H. M. N. (2013) ‘Alkali and enzymatic delignification of sugarcane bagasse to expose cellulose polymers for saccharification and bio-ethanol production’, Industrial Crops and Products, 44, pp. 488–495. doi: 10.1016/j.indcrop.2012.10.005.
Brienzo, M. et al. (2015) ‘Relationship between physicochemical properties and enzymatic hydrolysis of sugarcane bagasse varieties for bioethanol production’, New Biotechnology, 32(2), pp. 253–262. doi: 10.1016/j.nbt.2014.12.007.
Cardona, C. A., Quintero, J. A. and Paz, I. C. (2010) ‘Production of bioethanol from sugarcane bagasse: Status and perspectives’, Bioresource Technology, 101(13), pp. 4754–4766. doi: 10.1016/j.biortech.2009.10.097.
Córdova, A. et al. (2022a) ‘Recent Advances in the Application of Enzyme Processing Assisted by Ultrasound in Agri-Foods: A Review’, Catalysts, 12(1). doi: 10.3390/catal12010107.
Córdova, A. et al. (2022b) ‘Recent Advances in the Application of Enzyme Processing Assisted by Ultrasound in Agri-Foods: A Review’, Catalysts, 12(1). doi: 10.3390/catal12010107.
Furlan, F. F. et al. (2012) ‘Assessing the production of first and second generation bioethanol from sugarcane through the integration of global optimization and process detailed modeling’, Computers and Chemical Engineering, 43, pp. 1–9. doi: 10.1016/j.compchemeng.2012.04.002.
Furlan, F. F. et al. (2013) Bioelectricity versus bioethanol from sugarcane bagasse: is it worth being flexible?, Biotechnology for Biofuels. doi: 10.1186/1754-6834-6-142.
Galadima, A. and Muraza, O. (2019) ‘Zeolite catalyst design for the conversion of glucose to furans and other renewable fuels’, Fuel, 258. doi: 10.1016/j.fuel.2019.115851.
Gomez Del Pulgar, E. M. and Saadeddin, A. (2014) ‘The cellulolytic system of thermobifida fusca’, Critical Reviews in Microbiology, 40(3), pp. 236–247. doi: 10.3109/1040841X.2013.776512.
Heriyadi, P. and Kusnandar, F. (2014) Prinsip Teknik Pangan. 1st edn. Banten: Penerbit Universitas Terbuka.
Lakhundi, S., Siddiqui, R. and Khan, N. A. (2015) ‘Cellulose degradation: A therapeutic strategy in the improved treatment of Acanthamoeba infections’, Parasites and Vectors, 8(1). doi: 10.1186/s13071-015-0642-7.
Nasution, M. H., Lelinasari, S. and Kelana, M. G. S. (2022) ‘A review of sugarcane bagasse pretreatment for bioethanol production’, in IOP Conference Series: Earth and Environmental Science. IOP Publishing Ltd. doi: 10.1088/1755-1315/963/1/012014.
Rabelo, S. C. et al. (2011) ‘Production of bioethanol, methane and heat from sugarcane bagasse in a biorefinery concept’, Bioresource Technology, 102(17), pp. 7887–7895. doi: 10.1016/j.biortech.2011.05.081.
Restiawaty, E. et al. (2020) ‘Bioethanol production from sugarcane bagasse using neurospora intermedia in an airlift bioreactor’, International Journal of Renewable Energy Development, 9(2), pp. 247–253. doi: 10.14710/ijred.9.2.247-253.
Rocha, G. J. M. et al. (2012) ‘Steam explosion pretreatment reproduction and alkaline delignification reactions performed on a pilot scale with sugarcane bagasse for bioethanol production’, Industrial Crops and Products, 35(1), pp. 274–279. doi: 10.1016/j.indcrop.2011.07.010.
Saputro, E. A. et al. (2021) ‘Mass Balance Analysis of Bioethanol Production from Petai Peel (Parkia speciosa) through Enzymatic Process’, International Journal of Eco-Innovation in Science and Engineering, 2(02), pp. 30–33. doi: 10.4186/ijeise.upnjatim.ac.id/E-ISSN.
Sarkar, N. et al. (2012) ‘Bioethanol production from agricultural wastes: An overview’, Renewable Energy, 37(1), pp. 19–27. doi: 10.1016/j.renene.2011.06.045.
Sritrakul, N., Nitisinprasert, S. and Keawsompong, S. (2017) ‘Evaluation of dilute acid pretreatment for bioethanol fermentation from sugarcane bagasse pith’, Agriculture and Natural Resources, 51(6), pp. 512–519. doi: 10.1016/j.anres.2017.12.006.
Trisakti, B. and br Silitonga, Y. (2015) ‘PEMBUATAN BIOETANOL DARI TEPUNG AMPAS TEBU MELALUI PROSES HIDROLISIS TERMAL DAN FERMENTASI SERTA RECYCLE VINASSE (PENGARUH KONSENTRASI TEPUNG AMPAS TEBU, SUHU DAN WAKTU HIDROLISIS)’, Jurnal Teknik Kimia USU, 4(3).
Velmurugan, R. and Muthukumar, K. (2011) ‘Utilization of sugarcane bagasse for bioethanol production: Sono-assisted acid hydrolysis approach’, Bioresource Technology, 102(14), pp. 7119–7123. doi: 10.1016/j.biortech.2011.04.045.
Wang, B. et al. (2022) ‘Enhancement of sugar release from sugarcane bagasse through NaOH-catalyzed ethylene glycol pretreatment and water-soluble sulfonated lignin’, International Journal of Biological Macromolecules, 221, pp. 38–47. doi: 10.1016/j.ijbiomac.2022.08.193.
Wong, Y. C. and Sanggari, V. (2014) ‘Bioethanol production from sugarcane bagasse using fermentation process’, Oriental Journal of Chemistry, 30(2), pp. 507–513. doi: 10.13005/ojc/300214.
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Copyright (c) 2026 Erwan Adi Saputro, Adithya Daffa Rabbani, Miranda Annisa Anwar, Wiliandi Saputro, Intan Suhada Azmi, Ardika Nurmawati
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