UJI DYNAMIC BINDING CAPACITY KOLOM MONOLITH POLY (MATE-CO-VBC-CO-EDMA) PADA SAMPEL BIOMOLEKUL BOVINE SERUM ALBUMIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI
Keywords:
Polimer, Dynamic Binding Capacity, Protein, Monolith
Abstract
Kolom monolith poly(MATE-co-VBC-co-EDMA) dengan %T 35, dan %C 50 merupakan komposisi total monomer dan komposisi cross linker yang ideal, yaitu mempunyai kestabilan mekanis dan mekanis yang baik, molecular recognitioan sites memadai (ditandai dengan binding capacity 22,208 mg mL−1), serta proporsi flow-trough pores dan mesopores seimbang yaitu 24,24% dan 44,32%. Kolom Monolith %T 35% dan %C 50% mampu memisahkan secara akurat sampel biomolekul oligo(dT12-dT18) dan fragmen DNA dengan resolusi masing-masing 1,4 dan 1,0. Dari aplikasi model sampel oligo(dT12-dT18) dan fragmen DNA , pada pemisahan oligo(dT12-dT18) mekanisme pemisahan adalah penukar ion, sedangkan pada pada DNA termetilasi mekanisme pemisahan adalah interaksi hidrofobik, sehingga monolith poly(MATE-co-VBC-co-EDMA). Memberikan dua mekanisme pemisahan yang berbeda (dual-mode) pada aplikasisampel-sampel DNA
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Li Y., Huang C., Zheng J., Qi H. (2012) Electrogenerated chemiluminescence biosensing method for the detection ofDNA methylation and assay of the methyltransferase activityBiosensors and Bioelectronics 38 407–410.
Svec. F. (2010) Porous polymer monoliths: Amazingly wide variety of techniques enabling their preparation Journal of Chromatography A, 1217 902–924.
Nischang. I, Svec. F., Fréchet .J (2009)., Effect of capillary cross-section geometry and size on the separation of proteins in gradient mode using monolithic poly(butyl methacrylate-co-ethylenedimethacrylate) columns J. Chromatogr. A 1216 2355–236.
Chen. M.L., Liu. Y.L., Xing. X.W., Zhou. X., Feng. Y.Q., ,* Yuan.B.Q., (2013) Preparation of a Hyper-cross-linked Polymer Monolithic Column and Its Application to the Sensitive Determination of Genomic DNA MethylationChem. Eur. J. 2013, 19, 1035 – 1041
Cabera, K., Lubda, D., Eggenweiler, H.M., Minakuchi, H., Nakanishi, K., (2000), A new monolithic-type HPLC column for fast separations, Journal of High Resolution Chromatography, 23, 93–99
Hsieh, T. F. dan R. L. Fischer. (2005). Biology of chromatin dynamics. Annual Review of Plant Biology 56: 327-351.
Falck, E., Groenhagen, A., Muhlish, J., Hempel, G., Wunsch, B., (2012) , Genome-wide DNA methylation level analysis by micellar elektrokinetic chromatography and laser –induced fluorescence detection after treatment of cell lines with azacytidine and antifolates, Analytical Biochemistry, 421, 439-445
Fu, L.M., Lin, C.H., (2004), High-resolution DNA separation in microcapillary electrophoresis chips utilizing double-L injection techniques, Electrophoresis, 25, 3652-3659.
Hjerten, S., Liao, J.L, Zhang, R., (1989), High-performance liquid chromatography on continuous polymer beds, Journal of Chromatography, 473, 273–275.
Huber, C.G., Oefner, P.J., Preuss, E., Bonn, G.K., (1993), High-resolution liquid chromatography of DNA fragments on non-porous poly(styrene-divinylbenzene) particles, Nucleic Acids Research, 21, 1061-1066.
K. D. Robertson, Nat. Rev. Genet. (2005), 6, 597.
Kim, S.J., Kelly, W.K., Fu, A., Haines, K., Hoffman, A, Zheng, T, Zhu, Y., (2011), Genomewide methylation analysis identifies involvement of TNF-a mediated cancer pathways in prostate cancer, Cancer Letters, 302, 47–53.
Lubbad. S., Mayr., B., Huber, C.G., Buchmeiser, M.R., (2002), Micropreparative fractionation of DNA fragments on metathesis-based monoliths: influence of stoichiometry on separation, Journal of Chromatography A, 959, 121-129
Lu.YQ, Lin.Z.X., Svec. F (2012) Hypercrosslinked large surface area porous polymer monoliths for hydrophilic interaction liquid chromatography of small molecules featuring zwitterionic functionalities attached to gold nanoparticle sheld in layered structure. Anal Chem 84: 8457–8460.
Lubbad, S., Mayr, B., Huber, C.G., Buchmeiser, M.R., 2002, Micropreparative fractionation of DNA fragments on metathesis-based monoliths: influence of stoichiometry on separation, Journal of Chromatography A, 959, 121-129.
Morris, M.R., Ricketts, C. J., Gentle, D., McRonald, F., Carli, N., Khalili, H., Brown, M., Kishida, T., Yao, M., Banks, R.E., Clarke, N,. Latif, F., Maher, E. R., 2011, Genome-wide methylation analysis identifies epigenetically inactivated candidate tumour suppressor genes in renal cell carcinoma, Oncogene 30, 1390–1401.
Nakanishi, K., Soga, N., (1991), Phase separation in gelling silica–organic polymer solution: systems containing Ppoly(sodium styrenesulfonate), Journal of American Ceramic Society, 74, 2518
Nischang I, Teasdale I, Brüggemann. O(2010), Towards porous polymer monoliths for the efficient, retention-independent performance in the isocratic separation of small molecules by means of nano-liquid chromatography Journal of Chromatography A: 7514–7522
Plass, C. and P. D. Soloway. (2002). DNA methylation, imprinting, and cancer. European Journal of Human Genetics 10: 6-16.
Sabarudin, A.,(2012), Development of monolithic micro-extraction system for online collection/ concentration of trace elements: study on the substances circulation in the hydrosphere , JSPS report (unpublished).
Sabarudin, A., Huang, J., Sakagawa, S., Umemura, T., (2012), Preparation of Methacrylate-Based Anion-Exchange Monolithic Microbore Column for Chromatographic Separation of DNA Fragments and Oligonucleotides, Analytica Chimica Acta, 736, 108-114.
Sabarudin, A., Shu, S., Umemura, T., (2013), Microbore Monolithic Microreactor for High-Speed Suzuki-
Miyaura Cross-Coupling Reaction, AngewandteChemie (in submission).
Sabarudin, A., Rahmi, D., Takasaki, Y., Umemura, T., (2010), Development of monolithic chelating adsorbent for solid phase microextraction of trace elements in water samples, Annual Meeting of Japan Society For Analytical Chemistry, 15-17 September, Sendai, Japan.
Scuteri, A., Sanna, S., Chen, W. M., Uda, M., Albai, G., Strait, et al , (2007), Genome-Wide Association Scan Shows Genetic Variants in the FTO Gene Are Associated with Obesity-Related Traits, J. PLoS Genetics, 3, e115.
Shabo, A., (2008), Integrating genomics into clinical practice: standards and regulatory challenges, Current opinion in molecular therapeutics, 10, 267–272.
Shu, S., Kobayashi, H., Kojima, N., Sabarudin, A., Umemura, T.,(2011), Preparation and characterization of lauryl methacrylate-based monolithic microbore column for reversed-phase liquid chromatography, Journal of Chromatography A, 1218, 5228-5234.
Shu, S., Kobayashi, H., Okubo, M., Sabarudin, A., Butsugan, M., Umemura, T., (2012), Chemical anchoring of lauryl methacrylate-based reversed phase monolith to 1/16″ o.d. polyetheretherketone tubing, Journal of Chromatography A, 1242, 59-66.
Svec, F., (2004), Porous monoliths: emerging stationary phases for HPLC and related methods, LCGC LC Column Technology Supplement, June, 18–21
Takasaki, Y., Sakagawa, S., Inagaki, K., Fujii, S.I., Sabarudin, A., Umemura, T., Haraguchi, H., (2012), Development of salt-tolerance interface for an high performance liquid chromatography/inductively coupled plasma mass spectrometry system and its application to accurate quantification of DNA sample, Analytica Chimica Acta ,713, 23.
Toyota, M., Suzuki, H., Sasaki, Y., Maruyama, R., Imai, K., Shinomura, Y., Tokino, T., (2008), Epigenetic silencing of microRNA-34b/c and B-cell translocation gene 4 is associated with CpG island methylation in colorectal cancer, Cancer Research, 68, 4123.
Ueki, Y., Umemura, T., Li, J., Odake, T., Tsunoda, K., (2004), Preparation and application of methacrylate-based cation-exchange monolithic columns for capillary ion chromatography, Analytical Chemistry ,76, 7007-7012.
Umemura, T., Ueki, Y., Tsunoda, K-I., Katakai, A., Tamada, M., Haraguchi, H., (2006), Preparation and characterization of methacrylate-based semi-micro monoliths for high-throughput bioanalysis, Analytical and Bioanalytical Chemistry, 386, 566-571.
Urban J, Svec F, Frechet JMJ (2010) Efficient separation of small molecules using a large surface area hypercrosslinked monolithic polymer capillary column. Anal Chem 82: 1621–1623.
Yang, I., Fortin, M.C., Richardson, J. R., Buckley, B., (2011), Fused–core silica column ultraperformance liquid chromatography–ion trap tandem mass spectrometry for determination of global DNA methylation status, Analytical Biochemistry, 409, 138-14.
Yin, H., Zhou, Y., Xu, Z., Chen, L., Zhang, D., Ai, S., (2013), An electrochemical assay for DNA methylation , methyltransferase activity and inhibitor screening based on mpethyl binding domain protein, Biosensors and Bioelectronics, 41, 492-497.
Li Y., Huang C., Zheng J., Qi H. (2012) Electrogenerated chemiluminescence biosensing method for the detection ofDNA methylation and assay of the methyltransferase activityBiosensors and Bioelectronics 38 407–410.
Svec. F. (2010) Porous polymer monoliths: Amazingly wide variety of techniques enabling their preparation Journal of Chromatography A, 1217 902–924.
Nischang. I, Svec. F., Fréchet .J (2009)., Effect of capillary cross-section geometry and size on the separation of proteins in gradient mode using monolithic poly(butyl methacrylate-co-ethylenedimethacrylate) columns J. Chromatogr. A 1216 2355–236.
Chen. M.L., Liu. Y.L., Xing. X.W., Zhou. X., Feng. Y.Q., ,* Yuan.B.Q., (2013) Preparation of a Hyper-cross-linked Polymer Monolithic Column and Its Application to the Sensitive Determination of Genomic DNA MethylationChem. Eur. J. 2013, 19, 1035 – 1041
Published
2021-10-31
How to Cite
Eko Malis, & DIMAS PRIAGUNG BANAR S. (2021). UJI DYNAMIC BINDING CAPACITY KOLOM MONOLITH POLY (MATE-CO-VBC-CO-EDMA) PADA SAMPEL BIOMOLEKUL BOVINE SERUM ALBUMIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI. Jurnal Crystal : Publikasi Penelitian Kimia Dan Terapannya, 3(2), 6-13. https://doi.org/10.36526/jc.v3i2.1806
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