OPTIMASI ZAT PENGATUR TUMBUH TERHADAP REGENERASI TALAS KETAN MERAH SECARA IN VITRO

Fitri Damayanti; Acep Musliman

doi:10.36526/biosense.v9i2.7648

Authors

Fitri Damayanti Universitas Indraprasta PGRI
Acep Musliman Universitas Indraprasta PGRI

DOI:

https://doi.org/10.36526/biosense.v9i2.7648

Keywords:

Colocasia esculenta, kultur jaringan, induksi, regenerasi, zat pengatur tumbuh

Abstract

Talas (Colocasia esculenta L. Schott) merupakan salah satu tanaman pangan tropis dengan nilai gizi tinggi yang berperan penting dalam ketahanan pangan lokal maupun global. Namun, produktivitas tanaman ini sering terhambat oleh keterbatasan bibit berkualitas, rentan terhadap serangan patogen, serta siklus perbanyakan vegetatif yang relatif lambat. Teknik kultur jaringan, khususnya melalui induksi dan regenerasi secara in vitro, menawarkan solusi untuk menghasilkan planlet talas dalam jumlah besar dan seragam. Zat pengatur tumbuh (ZPT) berperan krusial dalam mengarahkan proses morfogenesis, baik pada tahap induksi kalus maupun regenerasi planlet. Penelitian ini bertujuan untuk mengkaji efektivitas berbagai kombinasi ZPT, yaitu NAA, BAP, dan kinetin dalam menginduksi tunas dan regenerasi planlet talas. Metode penelitian melibatkan kultur eksplan talas pada media Murashige dan Skoog (MS) dengan variasi konsentrasi ZPT. Kombinasi ZPT terbaik adalah BAP 1,0 mg/L + NAA 0,5 mg/L menghasilkan waktu induksi tunas tercepat dan jumlah tunas terbanyak. Perlakuan media MS yang hanya diberi NAA tunggal terbukti paling efektif untuk induksi perakaran, dengan konsentrasi terbaik untuk induksi perakaran adalah 1,0 mg/L. Penelitian ini diharapkan memberikan kontribusi terhadap pengembangan teknik perbanyakan massal talas yang efisien, mendukung konservasi plasma nutfah, serta meningkatkan produktivitas pertanian.

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