PENGUJIAN PERFORMA ALAT SPECTRONIC 20D+ DENGAN LARUTAN KALIUM PERMANGANAT (KMNO4)
DOI:
https://doi.org/10.36526/jc.v7i2.5992Keywords:
Spectonic 20D+, Equipment performance, Standard curve, Potassium permanganateAbstract
The Spectronic 20D+ is a simple spectophotometer that is still widely used in laboratories for both research and practicum activities. In order to ensure the continued accuracy of measurements, it is imperative that this instrument undergoes rigorous testing to ascertain its performance. The selection of potassium permanganate (KMnO4) as the testing solution is particularly salient in this context, given its notable advantages over conventional alternatives such as cobalt (II) chloride and potassium dichromate. These alternatives, while widely employed, are associated with a higher degree of risk and cost. The objective of this study is to ascertain the performance of the Spectronic 20D+ device when utilized with a KMnO4 solution. In this study, two units of Spectronic 20D+ were utilized, with one unit receiving a filter cleaning treatment only (PF), and the other undergoing a filter cleaning and replacement of the wolfram lamp (PFL). The maximum wavelength test yielded a value of 515 nanometers on both devices prior to the initiation of the repair process. The results of measuring the standard solution show that there is an increase in the amount of light absorbed, the slope of the line, and the coefficient of determination (R²). These were statistically tested with significantly different results on both tools after the repair process. This finding indicates that the measurement outcomes of the KMnO4 solution can serve as a reliable metric for evaluating the performance of the device before and after the repair process. Furthermore, following the restoration of the tool, the optimal linear range for KMnO4 concentration measurement was ascertained, ranging from 0 to 25 ppm. This determination was informed by the highest R2 value and the outcomes of cuvette correction testing, which yielded a consistent molar permissivity significance value as determined by the Duncan test for the five cuvettes utilized in this study.
References
Adeeyinwo, C.E., Okorie, N.N. and Idowu, G.O. (2013) ‘Basic calibration of UV/visible spectrophotometer’, International Journal of Science and Technology, 2(3), pp. 247–251.
Agbangba, C.E. et al. (2024) ‘On the use of post-hoc tests in environmental and biological sciences: A critical review’, Heliyon, 10(3), p. e25131. Available at: https://doi.org/https://doi.org/10.1016/j.heliyon.2024.e25131.
Al-kassab, M. and Majeed, A. (2022) ‘THE USE OF TWO-SAMPLE t-TEST IN THE REAL DATA’, Advances and Applications in Statistics, 81, pp. 13–22. Available at: https://doi.org/10.17654/0972361722071.
Dadi, M. and Yasir, M. (2022) ‘Spectroscopy and Spectrophotometry: Principles and Applications for Colorimetric and Related Other Analysis’, in. Available at: https://doi.org/10.5772/intechopen.101106.
El-Didamony, A.M., Saad, M.Z. and El-Shaprawy, D.S. (2013) ‘Direct and indirect spectrophotometric determination of some selected antibiotics using potassium permanganate’, Main Group Chemistry, 12(2), pp. 139–152. Available at: https://doi.org/10.3233/MGC-130096.
Farell, G. et al. (2023) ‘Prediction Analysis of Laboratory Equipment Depreciation Using Supervised Learning Methods’, TEM Journal, 12, pp. 1525–1532. Available at: https://doi.org/10.18421/TEM123-33.
Hao, L. et al. (2023) ‘Determination of trace potassium permanganate in tap water by solid phase extraction combined with spectrophotometry’, Heliyon, 9, p. e13587. Available at: https://doi.org/10.1016/j.heliyon.2023.e13587.
Hartonen, K. and Riekkola, M.-L. (2017) ‘Chapter 2 - Water as the First Choice Green Solvent’, in F. Pena-Pereira and M.B.T.-T.A. of G.S. in S.P. Tobiszewski (eds). Elsevier, pp. 19–55. Available at: https://doi.org/https://doi.org/10.1016/B978-0-12-805297-6.00002-4.
Hasibuan, N., Yurmaini, Y. and Erliyanti, E. (2023) ‘Pengaruh Perubahan Lingkungan Terhadap Kinerja Karyawan Pada Bank Syariah Indonesia’, Expensive: Jurnal Akuntansi dan Keuangan, 2(1), pp. 136–148. Available at: https://doi.org/10.24127/exclusive.v2i1.3697.
Hervey, J.R.D. et al. (2022) ‘A dual compartment cuvette system for correcting scattering in whole-cell absorbance spectroscopy of photosynthetic microorganisms.’, Photosynthesis research, 151(1), pp. 61–69. Available at: https://doi.org/10.1007/s11120-021-00866-8.
Irawan, A. (2019) ‘Kalibrasi Spektrofotometer Sebagai Penjaminan Mutu Hasil Pengukuran dalam Kegiatan Penelitian dan Pengujian’, Indonesian Journal of Laboratory, 1(2), p. 1. Available at: https://doi.org/10.22146/ijl.v1i2.44750.
Ismail, R. and Prihatini, E. (2023) ‘Tata Kelola Bahan Kimia di Laboratorium dengan Sistem Dokumen dan Perangkat Lunak Chemical Inventory Management System (CIMS) Berbasis Excel’, Indonesian Journal of Laboratory; Edisi Khusus 2023DO - 10.22146/ijl.v0i3.82022 [Preprint]. Available at: https://jurnal.ugm.ac.id/ijl/article/view/82022.
Kim, T. (2015) ‘T test as a parametric statistic’, Korean Journal of Anesthesiology, 68, p. 540. Available at: https://doi.org/10.4097/kjae.2015.68.6.540.
Lahm, H. (2004) ‘Performance verification of UV–VIS spectrophotometer’, Analytical Method Validation and Instrument Performance Verification [Preprint].
Nandiyanto, A.B.D., Ragadhita, R. and Aziz, M. (2023) ‘How to Calculate and Measure Solution Concentration using UV-Vis Spectrum Analysis: Supporting Measurement in the Chemical Decomposition, Photocatalysis, Phytoremediation, and Adsorption Process’, Indonesian Journal of Science and Technology, 8(2), pp. 345–362. Available at: https://doi.org/10.17509/ijost.v8i2.57783.
Nuhu, A., Sallau, M.. and Tukur, B. (2015) ‘Fixed sized simplex optimization of spectrophotometric method for the quantitative determination of diclofenac in pharmaceutical preparations’, International Journal of Advanced Research, 3, pp. 234–245.
Pradhan, S.K. and Tarafder, P.K. (2016) ‘A Scheme for Performance Evaluations of UV–Visible Spectrophotometer by Standard Procedures Including Certified Reference Materials for the Analysis of Geological Samples’, MAPAN, 31(4), pp. 275–281. Available at: https://doi.org/10.1007/s12647-016-0184-2.
Pramudya, L., Kumara, I.N.S. and Divayana, Y. (2024) ‘Peranan Kalibrasi Pada Alat Ukur : Literature Review’, Majalah Ilmiah Teknologi Elektro, 23(1), p. 37. Available at: https://doi.org/10.24843/mite.2024.v23i01.p05.
Prihatini, E. et al. (2023) ‘Uji Performa Alat Vakum Tekan Termodifikasi Untuk Impregnasi Kayu’, Jurnal Pengelolaan Laboratorium Pendidikan; Vol.5, No.2, Juli 2023DO - 10.14710/jplp.5.2.75-82 [Preprint]. Available at: https://ejournal2.undip.ac.id/index.php/jplp/article/view/15953.
Thompson, J. (2006) ‘A Simple Method for Rapidly Obtaining Absorption Spectra with a Spectronic-20D+ Spectrophotometer’, Journal of Chemical Education - J CHEM EDUC, 83(6), p. 913. Available at: https://doi.org/10.1021/ed083p913.
Workneh, S.M. et al. (2024) ‘Low-cost visible spectrophotometer for detecting absorption and emission in metallic blends of colorful samples solution’, Results in Optics, 16, p. 100703. Available at: https://doi.org/https://doi.org/10.1016/j.rio.2024.100703.
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