SPECTROSCOPIC CHARACTERIZATION AND UV-PROTECTIVE POTENTIAL OF ARCANGELISIA FLAVA EXTRACT
DOI:
https://doi.org/10.36526/jc.v7i2.5712Keywords:
Arcangelisia flava, UV-Vis spectroscopy, FTIR spectroscopy, flavonoids, UV protectionAbstract
The increasing demand for natural UV-protective agents has led to the exploration of plant-based bioactive compounds. Yellow root (Arcangelisia flava) extract is traditionally used in medicine and contains flavonoids, alkaloids, and terpenoids with potential photoprotective properties. This study aimed to characterize the chemical composition of A. flava extract and evaluate its UV-absorbing properties using UV-Vis and FTIR spectroscopy. The extract was obtained through maceration with ethanol, followed by spectroscopic analysis. The UV-Vis spectrum (200–400 nm) revealed multiple absorption peaks, particularly at 270 nm, 292 nm, 306 nm, 340 nm, 354 nm, 360 nm, and 396 nm, indicating the presence of UV-absorbing compounds. FTIR analysis confirmed the presence of hydroxyl (-OH), carbonyl (C=O), and aromatic (C=C) functional groups, which are characteristic of flavonoids and other bioactive compounds. These findings suggest that A. flava extract has potential as a natural UV-protective agent. Further studies on compound isolation, fractionation, and photostability assessments are required to optimize its purity and effectiveness in sunscreen formulations.
References
Aguilera, J. et al. (2021) ‘Booster Effect of a Natural Extract of Polypodium leucotomos (Fernblock®) That Improves the UV Barrier Function and Immune Protection Capability of Sunscreen Formulations’, Frontiers in Medicine, 8. Available at: https://doi.org/10.3389/fmed.2021.684665.
Baranović, G. and Šegota, S. (2018) ‘Infrared spectroscopy of flavones and flavonols. Reexamination of the hydroxyl and carbonyl vibrations in relation to the interactions of flavonoids with membrane lipids’, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 192, pp. 473–486. Available at: https://doi.org/10.1016/j.saa.2017.11.057.
Butnariu, M. (2023) ‘Physical Properties and Identification of Flavonoids by Ultraviolet-Visible Spectroscopy’, International Journal of Biochemistry & Physiology, 8(2). Available at: https://doi.org/10.23880/ijbp-16000239.
DEGÁSPARI, C.H. and WASZCZYNSKYJ, N. (2004) ‘PROPRIEDADES ANTIOXIDANTES DE COMPOSTOS FENÓLICOS’, Visão Acadêmica, 5(1). Available at: https://doi.org/10.5380/acd.v5i1.540.
Fahlman, B.M. and Krol, E.S. (2009) ‘UVA and UVB radiation-induced oxidation products of quercetin’, Journal of Photochemistry and Photobiology B: Biology, 97(3), pp. 123–131. Available at: https://doi.org/10.1016/j.jphotobiol.2009.08.009.
Fitriansyah, S.N. (2023) Antioxidant Activity and Sun Protection Factor of Different Parts of Sawo Walanda (Pouteria Campechiana (Kunth.) B.) Extract, Indonesian Journal of Pharmaceutical Science and Technology Journal Homepage. Available at: http://jurnal.unpad.ac.id/ijpst/.
Fotsing Yannick Stéphane, F. et al. (2022) ‘Extraction of Bioactive Compounds from Medicinal Plants and Herbs’, in Natural Medicinal Plants. IntechOpen. Available at: https://doi.org/10.5772/intechopen.98602.
Hamid, S. et al. (2012) ‘Comparative analysis of n-hexane and ethyl acetate extracts of Arisaema propinquum via Fourier Transform Infrared Spectroscopy (FTIR)’, International Journal of Food and Nutritional Sciences, 11(1), p. 2022. Available at: www.ijfans.org.
Hapsari, Y. et al. (2019) ‘Chemical Constituent and Antimalarial Activity based on Inhibition of Heme Polymerization from Water Extract of Yellow Root (Arcangelisia flava L. Merr)’, Chimica et Natura Acta, 7(3), p. 102. Available at: https://doi.org/10.24198/cna.v7.n3.26264.
Hari, N. and Nair, V.P. (2018) ‘FTIR Spectroscopic Analysis of Leaf Extract in Hexane in Jasminum Azoricum L’, International Journal of Scientific Research in Science and Technology, 4(8), pp. 170–172. Available at: www.ijsrst.com.
Indriarini, L. et al. (2021) ‘AKTIVITAS PERLINDUNGAN UV DAN ANTIOKSIDAN EKSTRAK KULIT JERUK (Citrus sinensis (L.) Osbeck) DALAM NANOGEL TABIR SURYA’, Jurnal Farmagazine, 8(2), p. 20. Available at: https://doi.org/10.47653/farm.v8i2.550.
José, M.T. de A.F. et al. (2016) ‘Flavonoids as photoprotective agents: A systematic review’, Journal of Medicinal Plants Research, 10(47), pp. 848–864. Available at: https://doi.org/10.5897/JMPR2016.6273.
Kaharap, A.D. et al. (2016) Uji efek antibakteri ekstrak batang akar kuning (Arcangelisia flava Merr.) terhadap bakteri Staphylococcus aureus dan Escherichia coli, Jurnal e-Biomedik (eBm).
Karim, F. et al. (2020) ‘UJI AKTIVITAS ANTIDIABETES AKAR KAYU KUNING (ARCANGELISIA FLAVA)’, Jurnal Kedokteran dan Kesehatan Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya, 7(3), pp. 35–40. Available at: https://doi.org/10.32539/JKK.V7I3.10190.
Kolina, J., Sumiwi, S.A. and Levita, J. (2019) ‘MODE IKATAN METABOLIT SEKUNDER DI TANAMAN AKAR KUNING (Arcangelisia flava L.) DENGAN NITRAT OKSIDA SINTASE’, FITOFARMAKA: Jurnal Ilmiah Farmasi, 8(1), pp. 45–52. Available at: https://doi.org/10.33751/jf.v8i1.1171.
Krakowska-Sieprawska, A. et al. (2022) ‘Modern Methods of Pre-Treatment of Plant Material for the Extraction of Bioactive Compounds’, Molecules, 27(3), p. 730. Available at: https://doi.org/10.3390/molecules27030730.
Lante, A., Tinello, F. and Nicoletto, M. (2016) ‘UV-A light treatment for controlling enzymatic browning of fresh-cut fruits’, Innovative Food Science & Emerging Technologies, 34, pp. 141–147. Available at: https://doi.org/10.1016/j.ifset.2015.12.029.
Marpaung, M.P. and Wahyuni, R.C. (2018) ‘Identifikasi Dan Penetapan Kadar Flavonoid Total Ekstrak Akar Kuning (Fibraurea chloroleuca Miers)’, Talenta Conference Series: Tropical Medicine (TM), 1(3), pp. 095–098. Available at: https://doi.org/10.32734/tm.v1i3.269.
Mayuresh Dev and Madhura Mukadam (2025a) ‘Functional group profiling of medicinal plants using FTIR spectroscopy’, World Journal of Biology Pharmacy and Health Sciences, 21(1), pp. 243–249. Available at: https://doi.org/10.30574/wjbphs.2025.21.1.0039.
Mayuresh Dev and Madhura Mukadam (2025b) ‘Functional group profiling of medicinal plants using FTIR spectroscopy’, World Journal of Biology Pharmacy and Health Sciences, 21(1), pp. 243–249. Available at: https://doi.org/10.30574/wjbphs.2025.21.1.0039.
Mergbi, M. et al. (2023) ‘Fast g-C3N4 sonocoated activated carbon for enhanced solar photocatalytic oxidation of organic pollutants through Adsorb & Shuttle process’, Ultrasonics Sonochemistry, 99, p. 106550. Available at: https://doi.org/10.1016/j.ultsonch.2023.106550.
Milutinov, J. et al. (2024) ‘The Potential of Natural Compounds in UV Protection Products’, Molecules, 29(22), p. 5409. Available at: https://doi.org/10.3390/molecules29225409.
Mushin M Shami, A. (2016) ‘Isolation and Identification of Alkaloids extracted from Local Plants in Malaysia’, Annals of Chromatography and Separation Techniques, 2(1), pp. 1–3. Available at: https://doi.org/10.36876/acst.1016.
Nafiah, S.R. et al. (2024) ‘Pengaruh Paparan Sinar Ultraviolet terhadap Kesehatan Kulit dan Upaya Pencegahannya : Tinjauan Literatur’, Scientific Journal (SCIENA), 3(3), pp. 185–1954. Available at: https://doi.org/https://doi.org/10.56260/sciena.v3i3.147.
Nunes, A.R. et al. (2018) ‘Use of Flavonoids and Cinnamates, the Main Photoprotectors with Natural Origin’, Advances in Pharmacological Sciences, 2018, pp. 1–9. Available at: https://doi.org/10.1155/2018/5341487.
de Oliveira, L.F.C. et al. (2002) ‘Vibrational spectroscopic study of brazilin and brazilein, the main constituents of brazilwood from Brazil’, Vibrational Spectroscopy, 28(2), pp. 243–249. Available at: https://doi.org/10.1016/S0924-2031(01)00138-2.
Panyakaew, J. et al. (2021) ‘Kaempferia Sp. Extracts as UV Protecting and Antioxidant Agents in Sunscreen’, Journal of Herbs, Spices & Medicinal Plants, 27(1), pp. 37–56. Available at: https://doi.org/10.1080/10496475.2020.1777614.
Pratama, M.R.F. (2016) ‘AKAR KUNING (Arcangelisia flava) SEBAGAI INHIBITOR EGFR : KAJIAN IN SILICO’, Jurnal Farmagazine, 3(1), pp. 6–16. Available at: https://doi.org/http://dx.doi.org/10.47653/farm.v3i1.1.
Pratama, M.R.F., Suratno, S. and Mulyani, E. (2018) ‘Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava)’, Borneo Journal of Pharmacy, 1(2), pp. 72–76. Available at: https://doi.org/10.33084/bjop.v1i2.367.
Rinaldi, S.E., Suryanto, S. and Widuri, S.A. (2017) ‘Informasi Perdagangan Akar Kuning di Pasar Tradisional Martapura dan Pasar Tradisional Rantau, Kalimantan Selatan’, Jurnal Sains dan Kesehatan, 1(8), pp. 434–439. Available at: https://doi.org/10.25026/jsk.v1i8.58.
Sahib, A.H.A., Al-Shareefi, E. and Hameed, I.H. (2019) ‘Detection of Bioactive Compounds of Vitex agnus-castus and Citrus sinensis Using Fourier-transform infrared spectroscopic profile and Evaluation of Its Anti-microbial Activity’, Indian Journal of Public Health Research & Development, 10(1), p. 954. Available at: https://doi.org/10.5958/0976-5506.2019.00184.0.
Smith, B.C. (2022) ‘The Infrared Spectra of Polymers, VI: Polymers With C-O Bonds’, Spectroscopy, pp. 15-19,27. Available at: https://doi.org/10.56530/spectroscopy.ly3071f5.
Subiandono, E. and Heriyanto, N.M. (2016) ‘Kajian Tumbuhan Obat Akar Kuning (Arcangelisia flava Merr.) di Kelompok Hutan Gelawan, Kabupaten Kampar, Riau’, Buletin Plasma Nutfah, 15(1), p. 43. Available at: https://doi.org/10.21082/blpn.v15n1.2009.p43-48.
Sukmawati, D.A.N. and Sembiring, Y.S. (2021) ‘Determination of Mefenamic Acid and Dexametashone in Instant Pegal Linu Herbal Medicine in Kediri by Using UV-Vis Spectro’, ALKIMIA : Jurnal Ilmu Kimia dan Terapan, 5(2), pp. 181–187. Available at: https://doi.org/10.19109/alkimia.v5i2.11275.
Suratno, S., Rizki, M.I. and Pratama, M.R.F. (2019) ‘In-Vitro Study of Antioxidant Activities from Ethanol Extracts of Akar Kuning (Arcangelisia flava)’, Jurnal Surya Medika, 4(2), pp. 66–71. Available at: https://doi.org/10.33084/jsm.v4i2.594.
Tata, H.L. et al. (2022) ‘Propagation of Bajakah and Akar Kuning to Support Bioprospecting of Traditional Medicine from Peat Swamp Forests: Prospect and Challenges’, Jurnal Manajemen Hutan Tropika (Journal of Tropical Forest Management), 28(2), pp. 112–118. Available at: https://doi.org/10.7226/jtfm.28.2.112.
Verdaguer, D. et al. (2017) ‘UV-A radiation effects on higher plants: Exploring the known unknown’, Plant Science, 255, pp. 72–81. Available at: https://doi.org/10.1016/j.plantsci.2016.11.014.
Wahyuni, S. and Marpaung, M.P. (2020) ‘PENENTUAN KADAR ALKALOID TOTAL EKSTRAK AKAR KUNING (Fibraurea chloroleuca Miers) BERDASARKAN PERBEDAAN KONSENTRASI ETANOL DENGAN METODE SPEKTROFOTOMETRI UV-VIS’, Dalton : Jurnal Pendidikan Kimia dan Ilmu Kimia, 3(2). Available at: https://doi.org/10.31602/dl.v3i2.3911.
Wahyuningsih, E. and Dessidianti, R. (2022) ‘Aplikasi FT-IR ATR Spektroskopi untuk Identifikasi Parasetamol pada Jamu Sediaan Serbuk’, Jurnal Camellia, 1(2), p. 56.
Wöhlecke, M. and Kovács, L. (2001) ‘OH− ions in Oxide Crystals’, Critical Reviews in Solid State and Materials Sciences, 26(1), pp. 1–86. Available at: https://doi.org/10.1080/20014091104161.
Yoandri, J. et al. (2022) ‘VALUASI TUMBUHAN AKAR KUNING (Arcangelisia flava Merr) DI DESA GOHONG KABUPATEN PULANG PISAU KALIMANTAN TENGAH’, Jurnal Hutan Tropika, 17(1), pp. 68–74. Available at: https://e-journal.upr.ac.id/index.php/JHT.
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